가슴수술 Things To Know Before You Buy
가슴수술 Things To Know Before You Buy
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Though we have not examined PneumaCult™ media to be used with murine epithelial cells, there are publications that report this application.
Consider preclinical test compounds and biologics for his or her abilities to modulate the immune system.
If working with Light Cell Dissociation Reagent (GCDR), the length of GCDR treatment must be optimized. Incubate organoids with GCDR for 8 -10 minutes at 37°C and make use of a P1000 pipette to gently pipette up and down, and visually inspect if organoids break up.
If cells do not dissociate well with trituration, go for an extended ACCUTASE™ incubation, nearly twelve minutes. If monolayers will not dissociate right after 12 minutes of ACCUTASE™ incubation and trituration, the differentiation was likely unsuccessful.
The STEMdiff™ system presents a standardized procedure for differentiating hPSCs into epithelial cells that may be later cultured in 2D or 3D formats with regards to the investigation demands.
There are 2 live-lifestyle morphology indicators forever differentiation and readiness for further probable characterization. These are generally:
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Take note: Continue to antibody staining if doing Stay cell Investigation right after this action. Alternatively, carry on to fixable dye staining and PFA fixation.
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To assess the regional specificity on the little vs large airway, it is possible to conduct the next assays:
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The next fluorochrome-conjugated antibodies and dyes are advised to facilitate analysis of gene-edited CD34+ HSPCs: